RegulonDB RegulonDB 11.2: Gene Form
   

glnA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

bipA glnA glnL unknown CRP NtrC NtrC NtrC NtrC Fis CRP CRP NtrC NtrC anti-anti-terminator anti-anti-terminator anti-terminator terminator TSS_4576 TSS_4576 TSS_4575 TSS_4575 TSS_4574 TSS_4574 TSS_4573 (cluster) TSS_4573 (cluster) TSS_4572 TSS_4572 TSS_4571 TSS_4571 TSS_4570 TSS_4570 TSS_4569 TSS_4569 TSS_4568 TSS_4568 TSS_4567 TSS_4567 typAp6 typAp6 typAp1 typAp1 glnAp1 glnAp1 glnAp2 glnAp2 TSS_4565 TSS_4565 TSS_4564 TSS_4564 TSS_4563 TSS_4563 TSS_4562 (cluster) TSS_4562 (cluster) TSS_4561 TSS_4561 TSS_4560 TSS_4560 TSS_4559 TSS_4559 TSS_4558 TSS_4558 TSS_4557 (cluster) TSS_4557 (cluster) TSS_4556 TSS_4556 TSS_4555 TSS_4555 TSS_4554 TSS_4554 TSS_4553 TSS_4553 TSS_4552 TSS_4552 TSS_4551 TSS_4551 TSS_4550 (cluster) TSS_4550 (cluster) TSS_4549 TSS_4549 TSS_4548 (cluster) TSS_4548 (cluster) glnLp glnLp

Gene      
Name: glnA    Texpresso search in the literature
Synonym(s): ECK3863, EG10383, b3870
Genome position(nucleotides): 4056625 <-- 4058034
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.91
External database links:  
ASAP:
 ABE-0012640
ECHOBASE:
 EB0378
ECOLIHUB:
 glnA
OU-MICROARRAY:
 b3870
STRING:
 511145.b3870
COLOMBOS: glnA


Product      
Name: glutamine synthetase
Synonym(s): GlnA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,membrane
Molecular weight: 51.904
Isoelectric point: 5.143
Motif(s):
 
Type Positions Sequence Comment
2 -> 469 SAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFFEEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYDRDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWNSSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAGQNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLSKNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLAYSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEAMDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREEDDRVRMTPHPVEFELYYSV UniProt: Glutamine synthetase.
14 -> 95 EVKFVDLRFTDTKGKEQHVTIPAHQVNAEFFEEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEP
90 -> 90 C UniProt: In Ref. 2; AAA23879..
103 -> 466 RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWNSSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAGQNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLSKNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLAYSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEAMDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREEDDRVRMTPHPVEFELY
108 -> 109 IA UniProt: In Ref. 2; AAA23879..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.2 - glutamine
  1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.3 - nitrogen metabolism
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016020 - membrane
molecular_function GO:0003824 - catalytic activity
GO:0046872 - metal ion binding
GO:0016874 - ligase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004356 - glutamate-ammonia ligase activity
GO:0042802 - identical protein binding
biological_process GO:0006542 - glutamine biosynthetic process
GO:0006807 - nitrogen compound metabolic process
GO:0019740 - nitrogen utilization
GO:0019676 - ammonia assimilation cycle
GO:0009314 - response to radiation
External database links:  
ALPHAFOLD:
 P0A9C5
DIP:
 DIP-9777N
ECOCYC:
 GLUTAMINESYN-MONOMER
ECOLIWIKI:
 b3870
INTERPRO:
 IPR014746
INTERPRO:
 IPR027302
INTERPRO:
 IPR036651
INTERPRO:
 IPR008147
INTERPRO:
 IPR008146
INTERPRO:
 IPR004809
INTERPRO:
 IPR001637
INTERPRO:
 IPR027303
PFAM:
 PF03951
PFAM:
 PF00120
PRIDE:
 P0A9C5
PROSITE:
 PS00182
PROSITE:
 PS00181
PROSITE:
 PS00180
REFSEQ:
 NP_418306
SMART:
 SM01230
SMR:
 P0A9C5
SWISSMODEL:
 P0A9C5
UNIPROT:
 P0A9C5


Operon      
Name: glnALG         
Operon arrangement:
Transcription unit        Promoter
glnLG
glnA
glnALG
glnA
glnALG


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, NtrC, Fis
Repressed by: CRP, NtrC


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4548 (cluster) 4056602 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4549 4056615 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4550 (cluster) 4056622 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4551 4057070 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4552 4057076 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4553 4057079 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4554 4057208 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4555 4057214 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4556 4057685 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4557 (cluster) 4057694 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4558 4057741 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4559 4057761 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4560 4058041 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4561 4058052 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4562 (cluster) 4058056 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4563 4058061 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4564 4058078 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4565 4058086 reverse nd [RS-EPT-CBR] [1]
  promoter typAp1 4058230 forward nd [COMP-AINF] [2]
  promoter typAp6 4058380 forward nd [COMP-AINF], [RS-EPT-CBR] [1], [2]
  promoter TSS_4567 4058771 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4568 4058776 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4569 4059121 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4570 4059139 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4571 4059223 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4572 4059230 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4573 (cluster) 4059232 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4574 4059387 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4575 4059523 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4576 4060087 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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