RegulonDB RegulonDB 11.2: Gene Form
   

glnG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

glnG glnL yshB TSS_4547 (cluster) TSS_4547 (cluster) TSS_4546 (cluster) TSS_4546 (cluster)

Gene      
Name: glnG    Texpresso search in the literature
Synonym(s): ECK3861, EG10385, b3868, glnT, ntrC
Genome position(nucleotides): 4053869 <-- 4055278
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.95
External database links:  
ASAP:
 ABE-0012632
CGSC:
 702
ECHOBASE:
 EB0380
ECOLIHUB:
 glnG
OU-MICROARRAY:
 b3868
STRING:
 511145.b3868
COLOMBOS: glnG


Product      
Name: DNA-binding transcriptional dual regulator NtrC
Synonym(s): GlnG, GlnT, NtrC, NtrC response regulator
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 52.255
Isoelectric point: 6.465
Motif(s):
 
Type Positions Sequence Comment
5 -> 119 IVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAI UniProt: Response regulatory.
6 -> 115 VWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALV
140 -> 306 IIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHRLNVIRV
142 -> 144 GEA UniProt: In Ref. 1; CAA28808..
168 -> 175 GESGTGKE UniProt: ATP.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.2 - glutamine
  2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.2 - activator
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.4 - complex regulation --> 3.1.2.4.3 - two component regulatory systems (external signal)
  3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003677 - DNA binding
GO:0005515 - protein binding
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0000156 - phosphorelay response regulator activity
GO:0043565 - sequence-specific DNA binding
biological_process GO:0006355 - regulation of transcription, DNA-templated
GO:0000160 - phosphorelay signal transduction system
GO:0006808 - regulation of nitrogen utilization
GO:0009399 - nitrogen fixation
Reference(s): [1] Lee J., et al., 2000
[2] Pahel G., et al., 1979
External database links:  
ALPHAFOLD:
 P0AFB8
DIP:
 DIP-9781N
ECOCYC:
 PROTEIN-NRI
ECOLIWIKI:
 b3868
INTERPRO:
 IPR003593
INTERPRO:
 IPR009057
INTERPRO:
 IPR010114
INTERPRO:
 IPR011006
INTERPRO:
 IPR025662
INTERPRO:
 IPR025943
INTERPRO:
 IPR025944
INTERPRO:
 IPR002078
INTERPRO:
 IPR001789
INTERPRO:
 IPR027417
INTERPRO:
 IPR002197
MODBASE:
 P0AFB8
PFAM:
 PF02954
PFAM:
 PF00158
PFAM:
 PF00072
PRIDE:
 P0AFB8
PRINTS:
 PR01590
PRODB:
 PRO_000022786
PROSITE:
 PS00675
PROSITE:
 PS00688
PROSITE:
 PS50045
PROSITE:
 PS50110
PROSITE:
 PS00676
REFSEQ:
 NP_418304
SMART:
 SM00382
SMART:
 SM00448
SMR:
 P0AFB8
SWISSMODEL:
 P0AFB8
UNIPROT:
 P0AFB8


Operon      
Name: glnALG         
Operon arrangement:
Transcription unit        Promoter
glnLG
glnA
glnALG
glnA
glnALG


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, NtrC, Fis
Repressed by: CRP, NtrC


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4546 (cluster) 4053777 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4547 (cluster) 4053810 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Lee J., Owens JT., Hwang I., Meares C., Kustu S., 2000, Phosphorylation-induced signal propagation in the response regulator ntrC., J Bacteriol 182(18):5188-95

 [2] Pahel G., Tyler B., 1979, A new glnA-linked regulatory gene for glutamine synthetase in Escherichia coli., Proc Natl Acad Sci U S A 76(9):4544-8

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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