RegulonDB RegulonDB 11.2: Gene Form
   

purN gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ppk purM purN upp PurR FNR FNR terminator anti-terminator anti-anti-terminator TSS_2782 (cluster) TSS_2782 (cluster) TSS_2781 (cluster) TSS_2781 (cluster) ppkp6 ppkp6 ppkp ppkp ppkp5 ppkp5 purMp purMp TSS_2780 TSS_2780 uppp uppp

Gene      
Name: purN    Texpresso search in the literature
Synonym(s): ECK2496, EG10799, ade, ade(c), b2500
Genome position(nucleotides): 2622234 --> 2622872
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.68
Reference(s): [1] Andersen PS., et al., 1992
[2] Smith JM., et al., 1987
[3] Watanabe W., et al., 1989
External database links:  
ASAP:
 ABE-0008232
CGSC:
 17623
ECHOBASE:
 EB0792
ECOLIHUB:
 purN
OU-MICROARRAY:
 b2500
STRING:
 511145.b2500
COLOMBOS: purN


Product      
Name: phosphoribosylglycinamide formyltransferase 1
Synonym(s): Ade, PurN
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 23.238
Isoelectric point: 5.741
Motif(s):
 
Type Positions Sequence Comment
1 -> 181 MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLERARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAGRLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGDSEDDITARVQTQEHAIYPLVI
11 -> 13 GSN UniProt: 5'-phosphoribosylglycinamide binding; Sequence Annotation Type: region of interest.
70 -> 70 E UniProt: Loss of homodimerization. No effect on activity..
89 -> 92 MRIL UniProt: 10-formyltetrahydrofolate binding; Sequence Annotation Type: region of interest.
106 -> 106 N UniProt: Loss of activity..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.2 - nucleotides --> 1.5.2.1 - purine biosynthesis
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016740 - transferase activity
GO:0016742 - hydroxymethyl-, formyl- and related transferase activity
GO:0004644 - phosphoribosylglycinamide formyltransferase activity
biological_process GO:0006164 - purine nucleotide biosynthetic process
GO:0006974 - cellular response to DNA damage stimulus
GO:0009058 - biosynthetic process
GO:0006189 - 'de novo' IMP biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [4] Morikis D., et al., 2001
External database links:  
ALPHAFOLD:
 P08179
ECOCYC:
 GART-MONOMER
ECOLIWIKI:
 b2500
INTERPRO:
 IPR036477
INTERPRO:
 IPR004607
INTERPRO:
 IPR002376
INTERPRO:
 IPR001555
MODBASE:
 P08179
PDB:
 3GAR
PDB:
 2GAR
PDB:
 1JKX
PDB:
 1GRC
PDB:
 1GAR
PDB:
 1CDE
PDB:
 1CDD
PDB:
 1C3E
PDB:
 1C2T
PFAM:
 PF00551
PRIDE:
 P08179
PRODB:
 PRO_000023643
PROSITE:
 PS00373
REFSEQ:
 NP_416995
SMR:
 P08179
UNIPROT:
 P08179


Operon      
Name: purMN         
Operon arrangement:
Transcription unit        Promoter
purMN


Transcriptional Regulation      
Display Regulation             
Repressed by: PurR, FNR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2780 2620986 reverse nd [RS-EPT-CBR] [5]
  promoter ppkp5 2622874 forward nd [COMP-AINF] [6]
  promoter ppkp6 2622990 forward nd [COMP-AINF] [6]
  promoter TSS_2781 (cluster) 2625007 forward nd [RS-EPT-CBR] [5]
  promoter TSS_2782 (cluster) 2625014 forward nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Andersen PS., Smith JM., Mygind B., 1992, Characterization of the upp gene encoding uracil phosphoribosyltransferase of Escherichia coli K12., Eur J Biochem 204(1):51-6

 [2] Smith JM., Daum HA., 1987, Identification and nucleotide sequence of a gene encoding 5'-phosphoribosylglycinamide transformylase in Escherichia coli K12., J Biol Chem 262(22):10565-9

 [3] Watanabe W., Sampei G., Aiba A., Mizobuchi K., 1989, Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis., J Bacteriol 171(1):198-204

 [4] Morikis D., Elcock AH., Jennings PA., McCammon JA., 2001, Proton transfer dynamics of GART: the pH-dependent catalytic mechanism examined by electrostatic calculations., Protein Sci 10(11):2379-92

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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