RegulonDB RegulonDB 11.2: Gene Form
   

aidB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

aidB yjfC yjfN AidB Ada TSS_5053 TSS_5053 aidBp2 aidBp2 aidBp1 aidBp1

Gene      
Name: aidB    Texpresso search in the literature
Synonym(s): ECK4183, EG11811, b4187
Genome position(nucleotides): 4414275 --> 4415900
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.31
External database links:  
ASAP:
 ABE-0013701
CGSC:
 18553
ECHOBASE:
 EB1759
ECOLIHUB:
 aidB
OU-MICROARRAY:
 b4187
STRING:
 511145.b4187
COLOMBOS: aidB


Product      
Name: putative acyl-CoA dehydrogenase AidB
Synonym(s): AidB, isovaleryl-CoA dehydrogenase and DNA-binding transcriptional repressor
Sequence: Get amino acid sequence Fasta Format
Regulator Family: AidB_like
Cellular location: cytosol
Molecular weight: 60.59
Isoelectric point: 7.892
Motif(s):
 
Type Positions Sequence Comment
2 -> 439 HWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLASIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHNLAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPLLSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFSVPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDAIGWLLGLEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQPLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPFVAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVL isovaleryl-CoA dehydrogenase activity
10 -> 167 NQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLASIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHNLAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPLLSDRYDS
181 -> 275 GMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFSVPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQD
182 -> 191 MGMTEKQGGS UniProt: FAD.
216 -> 218 FFS UniProt: FAD.

 
Classification:
Multifun Terms (GenProtEC)  
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003677 - DNA binding
GO:0005515 - protein binding
GO:0016491 - oxidoreductase activity
GO:0016627 - oxidoreductase activity, acting on the CH-CH group of donors
GO:0003995 - acyl-CoA dehydrogenase activity
GO:0008470 - isovaleryl-CoA dehydrogenase activity
GO:0043565 - sequence-specific DNA binding
GO:0042802 - identical protein binding
biological_process GO:0006974 - cellular response to DNA damage stimulus
GO:0045892 - negative regulation of transcription, DNA-templated
Note(s): Note(s): ...[more].
Reference(s): [1] Vasil'eva SV., et al., 2005
External database links:  
ALPHAFOLD:
 P33224
DIP:
 DIP-9078N
ECOCYC:
 EG11811-MONOMER
ECOLIWIKI:
 b4187
INTERPRO:
 IPR041504
INTERPRO:
 IPR036250
INTERPRO:
 IPR034184
INTERPRO:
 IPR009100
INTERPRO:
 IPR009075
INTERPRO:
 IPR006091
INTERPRO:
 IPR006089
MODBASE:
 P33224
PDB:
 3DJL
PDB:
 3U33
PFAM:
 PF02770
PFAM:
 PF18158
PFAM:
 PF00441
PRIDE:
 P33224
PRODB:
 PRO_000022072
PROSITE:
 PS00072
PROSITE:
 PS00073
REFSEQ:
 NP_418608
SMR:
 P33224
UNIPROT:
 P33224


Operon      
Name: aidB         
Operon arrangement:
Transcription unit        Promoter
aidB
aidB


Transcriptional Regulation      
Display Regulation             
Activated by: Ada
Repressed by: AidB, Lrp


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_5053 4415541 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Vasil'eva SV., Moshkovskaia EIu., 2005, [Quasi-adaptive response to alkylating agents in Escherichia coli: a new phenomenon], Genetika 41(5):607-13

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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