Notes For:
3-ketoacyl-CoA thiolase (FadA) is involved in the degradation of fatty acids via the β-oxidation cycle. It has broad chain-length specificity for substrates, but exhibits its highest activity with medium-chain substrates.
FadA and FadB form a multifunctional enzyme complex |CITS: [334745][6402028][3286611][8454629]| (data from E. coli B in |CITS: [7012144][6350283]|).
Overexpression of FadA and FadB can be utilized for the production of long-chain fatty acids in an engineered reversal of the β-oxidation cycle |CITS: [21832992][23656231]|.
Expression of the enzymes involved in β-oxidation is normally induced by fatty acids of chain length 14 and longer |CITS: [4861587][4886296][18317523]|. Regulation is at the transcriptional level and involves repression by FadR |CITS: [6271734]|.
OldA: oleate degradation A (old-30) |CITS: [4861587][4928881]|
FadA: fatty acid degradation A |CITS: [4563344]|
| Reference(s): |
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| [7] Pramanik A., Schulz H., 1983, Multienzyme complexes of fatty acid oxidation from Escherichia coli K12 and from a mutant with a defective L-3-hydroxyacyl coenzyme A dehydrogenase., Biochim Biophys Acta 750(1):41-6 |
| [8] Pauli G, Overath P, 1972, ato Operon: a highly inducible system for acetoacetate and butyrate degradation in Escherichia coli., Eur J Biochem, 29(3):553 10.1111/j.1432-1033.1972.tb02021.x |
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