RegulonDB RegulonDB 11.2: Operon Form
   

gspAB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: gspAB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: gspAB
Gene(s): gspB, gspA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [COMP-AINF] Inferred computationally without human oversight
Promoter
Name: gspAp3
+1: 3455495
Distance from start of the gene: 97
Sequence: attgggtgggttgtatattgacgttagtataaatagtattaatgccgcttgcatgaatcaAgcaaaatttatttatattag
Evidence: [EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [1] Francetic O., et al., 2000


Transcription unit          
Name: gspAB
Gene(s): gspB, gspA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [COMP-AINF] Inferred computationally without human oversight
Promoter
Name: gspAp2
+1: 3455510
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 112
Sequence: gttctaataaaatagattgggtgggttgtatattgacgttagtataaatagtattaatgcCgcttgcatgaatcaagcaaa
                               -35                       -10+1                   
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [1] Francetic O., et al., 2000
[2] Huerta AM., et al., 2003
TF binding sites (TFBSs)
[H-NS,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd H-NS repressor gspAp2 nd nd nd nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [1]


Transcription unit          
Name: gspAB
Gene(s): gspB, gspA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [COMP-AINF] Inferred computationally without human oversight
Promoter
Name: gspAp1
+1: 3455519
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 121
Sequence: acgatgtatgttctaataaaatagattgggtgggttgtatattgacgttagtataaatagTattaatgccgcttgcatgaa
                               -35                       -10+1                   
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [1] Francetic O., et al., 2000
[2] Huerta AM., et al., 2003
TF binding sites (TFBSs)
[H-NS,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd H-NS repressor gspAp1 nd nd nd nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [1]


Transcription unit       
Name: gspAB
Synonym(s): gspA-pioO
Gene(s): gspB, gspA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [COMP-AINF] Inferred computationally without human oversight
Promoter
Name: gspAp4
+1: 3455634
Sigma Factor: Sigma24 Sigmulon
Distance from start of the gene: 236
Sequence: atggaaataattcacattaaaaataatcgaccctatgcttataaatataatcaatatattGatggcagcatctttattatg
                                  -35                  -10  +1                   
Evidence: [COMP-AINF]
[COMP-AINF-POSITIONAL-IDENTIFICATION]
[COMP-AINF-SIMILAR-TO-CONSENSUS]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [2] Huerta AM., et al., 2003
[3] Rhodius VA., et al., 2005


Evidence    

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [COMP-AINF-POSITIONAL-IDENTIFICATION] Automated inference of promoter position

 [COMP-AINF-SIMILAR-TO-CONSENSUS] Automated inference based on similarity to consensus sequences


Reference(s)    

 [1] Francetic O., Belin D., Badaut C., Pugsley AP., 2000, Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion., EMBO J 19(24):6697-703

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Rhodius VA., Suh WC., Nonaka G., West J., Gross CA., 2005, Conserved and variable functions of the sigmaE stress response in related genomes., PLoS Biol 4(1):e2

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