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| Synonyms: BetI, BetI-choline |
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Summary:
"Betaine inhibitor," BetI, acts as a repressor of glycine betaine synthesis from choline [2] It is negatively autoregulated and coordinately represses transcription of the choline transporter and the operon related to the synthesis of glycine betaine. These divergent operons are expressed only under aerobic conditions and are induced by osmotic stress [4] BetI is an unusual repressor because it remains bound to DNA during induction. BetI forms a complex with DNA in the presence of choline, binding to a 20-bp-long sequence with dyad symmetry that overlaps betTp and betIp simultaneously [1] The position of the repressor gene within the operon is unusual too, as regulatory genes tend to be positioned at the end of operons and this gene is located at the beginning of the operon. BetI is still able to repress transcription at positions as far as -61 from the TSS, but in a weak manner [5] BetI belongs to the TetR/AcrR family of transcriptional regulators [1, 4] This protein composed of two domains, an amino-terminal domain that contains a potential helix-turn-helix DNA-binding motif and a carboxy-terminal domain involved in co-inducer recognition and dimerization [4]. Read more > |
| Transcription factor |  |
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| TF conformation(s): |
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| Evolutionary Family: | TetR/AcrR | |||||||||||||||||||||
| TFBs length: | 20 | |||||||||||||||||||||
| TFBs symmetry: | inverted-repeat | |||||||||||||||||||||
| Sensing class: | External sensing using transported metabolites | |||||||||||||||||||||
| Connectivity class: | Local Regulator | |||||||||||||||||||||
| Gene name: | betI | |||||||||||||||||||||
| Genome position: | 328747-329334 | |||||||||||||||||||||
| Length: | 588 bp / 195 aa | |||||||||||||||||||||
| Operon name: | betIBA | |||||||||||||||||||||
| TU(s) encoding the TF: |
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| Regulon |
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| Regulated gene(s) | betA, betB, betI, betT | ||||||
| Multifun term(s) of regulated gene(s) |
MultiFun Term (List of genes associated to the multifun term)
betaine biosynthesis (3)
osmotic pressure (3)
membrane (2)
Transcription related (1)
repressor (1)
Read more >
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| Regulated operon(s) | betIBA, betT | ||||||
| First gene in the operon(s) | betI, betT | ||||||
| Simple and complex regulons | ArcA,BetI,Cra | ||||||
| Simple and complex regulatory phrases | Regulatory phrase (List of promoters regulated by the phrase) | ||||||
| Transcription factor regulation |
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Transcription factor binding sites (TFBSs) arrangements
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| Functional conformation | Function | Promoter | Sigma factor | Central Rel-Pos | Distance to first Gene | Genes | Sequence | LeftPos | RightPos | Evidence | Confidence level (C: Confirmed, S: Strong, W: Weak) | References | |
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| BetI | repressor | betIp | Sigma70 | -30.0 | -57.0 | betI, betB, betA |
agacacatttTATATTGAACGTCCAATCAAtaaccgcttt
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329381 | 329400 | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-GSELEX], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] | C | [1], [1], [2], [2], [3], [3] | |
| BetI | repressor | betTp | Sigma70 | -30.0 | -72.0 | betT |
aaagcggttaTTGATTGGACGTTCAATATAaaatgtgtct
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329381 | 329400 | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-GSELEX], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] | C | [1], [1], [2], [2], [3], [3] |
| Evolutionary conservation of regulatory elements | |
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| Promoter-target gene evolutionary conservation |
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| Reference(s) |
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[EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins