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BtsR DNA-binding transcriptional dual regulator

Synonyms: BtsR, BtsR-Phosphorylated

Summary:
YehT has similarity to two-component system response regulator proteins [3]. Overproduction causes a drug resistance phenotype [3, 4].

Transcription factor

TF conformation(s):

Name	Conformation Type	TF-Effector Interaction Type	Apo/Holo Conformation	Evidence	Confidence level (C: Confirmed, S: Strong, W: Weak)	References
BtsR	Non-Functional		Apo	nd	nd	nd
BtsR-Phosphorylated	Functional		Holo	nd	nd	nd

Evolutionary Family:

LytTR

Sensing class:

External-Two-component systems

Connectivity class:

Local Regulator

Gene name:

Genome position:

2212243-2212962

Length:

720 bp / 239 aa

Operon name:

TU(s) encoding the TF:

Transcription unit	Promoter
btsSR

Regulon
Regulated gene(s)	aceE, aceF, btsT, csgA, csgB, csgC, csgD, csgE, csgF, csgG, cspC, lpd, mdh, pdhR, yobF
Multifun term(s) of regulated gene(s)	MultiFun Term (List of genes associated to the multifun term) carbon compounds (4) aceE, aceF, lpd, mdh pyruvate dehydrogenase (3) aceE, aceF, lpd glyoxylate degradation (3) aceE, aceF, mdh fimbri, pili (3) csgA, csgC, csgF Transcription related (3) csgD, cspC, pdhR membrane (2) btsT, csgF defense/survival (2) csgA, csgC activator (2) csgD, pdhR repressor (2) csgD, pdhR Porters (Uni-, Sym- and Antiporters) (1) btsT glycoprotein (1) csgB pilus (1) csgB Accessory Factors Involved in Transport (1) csgE Channel-type Transporters (1) csgG transcriptional level (1) cspC adaptations (1) cspC amino acids (1) lpd formyl-THF biosynthesis (1) lpd TCA cycle (1) mdh fermentation (1) mdh central intermediary metabolism (1) mdh glycolysis (1) pdhR operon (1) pdhR pH (1) yobF Read more >
Regulated operon(s)	btsT, csgBAC, csgDEFG, mdh, pdhR-aceEF-lpd, yobF-cspC
First gene in the operon(s)	btsT, csgB, csgD, mdh, pdhR, yobF
Simple and complex regulons	ArcA,BtsR,CRP,DpiA,FlhDC BasR,BolA,BtsR,CRP,CpxR,Cra,CsgD,FliZ,H-NS,IHF,MlrA,MqsA,OmpR,RcdA,RcsAB,RstA,ppGpp BasR,BtsR,CRP,CpxR,Cra,CsgD,FliZ,H-NS,IHF,MlrA,MqsA,OmpR,RcdA,RcsAB,RstA BtsR BtsR,CRP BtsR,CRP,Cra,FNR,PdhR BtsR,CpxR,CsgD,FliZ Read more >
Simple and complex regulatory phrases	Regulatory phrase (List of promoters regulated by the phrase) [BtsR,+](1) yjiYp [BtsR,-](8) csgBp, csgBp2, csgDp1, csgDp3, mdhp1, pdhRp, pdhRp2, yobFp1

Transcription factor regulation

Transcription factor binding sites (TFBSs) arrangements

	Functional conformation	Function	Promoter	Sigma factor	Central Rel-Pos	Distance to first Gene	Genes	Sequence	LeftPos	RightPos	Evidence	Confidence level (C: Confirmed, S: Strong, W: Weak)	References
	BtsR-Phosphorylated	repressor	csgBp	Sigma70	62.0	-31.0	csgB, csgA, csgC	aaatgatttaATTTCTTAAATGtacgaccagg nd	1103915	1103926	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	csgBp2	Sigma38	62.0	-31.0	csgB, csgA, csgC	aaatgatttaATTTCTTAAATGtacgaccagg nd	1103915	1103926	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	csgDp1	Sigma70	-25.0	-173.0	csgD, csgE, csgF, csgG	tttaacacttGATTTAAGATTTgtaatggcta nd	1103364	1103375	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	csgDp1	Sigma70	37.0	-112.0	csgD, csgE, csgF, csgG	tatattttacCCATTTAGGGCTgatttattac nd	1103303	1103314	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	csgDp3	Sigma38	-25.0	-173.0	csgD, csgE, csgF, csgG	tttaacacttGATTTAAGATTTgtaatggcta nd	1103364	1103375	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	csgDp3	Sigma38	37.0	-112.0	csgD, csgE, csgF, csgG	tatattttacCCATTTAGGGCTgatttattac nd	1103303	1103314	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	mdhp1	Sigma70	-145.0	-190.0	mdh	ccacctgttgGAATGTTGCGCTaatgcataag nd	3384453	3384464	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	mdhp1	Sigma70	-73.0	-118.0	mdh	ttaaatgcatAAACGCTAAACTtgcgtgacta nd	3384381	3384392	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	mdhp1	Sigma70	-49.0	-94.0	mdh	cgtgactacaCATTCTTGAGATgtggtcattg nd	3384357	3384368	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	pdhRp	Sigma70	-203.0	-261.0	pdhR, aceE, aceF, lpd	tgaattaaaaATCACAAAATTGgtaagtgaat nd	121826	121837	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	pdhRp	Sigma70	-188.0	-246.0	pdhR, aceE, aceF, lpd	aaaattggtaAGTGAATCGGTTcaattcggat nd	121841	121852	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	pdhRp2	Sigma38	-203.0	-261.0	pdhR, aceE, aceF, lpd	tgaattaaaaATCACAAAATTGgtaagtgaat nd	121826	121837	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	pdhRp2	Sigma38	-188.0	-246.0	pdhR, aceE, aceF, lpd	aaaattggtaAGTGAATCGGTTcaattcggat nd	121841	121852	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	activator	yjiYp	Sigma38	-62.0	-150.0	btsT	ccgcaaaactGACCGCTTACATCCCTAAAATAACCACTCAgttatttacc nd	4591415	4591444	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION]	C	[1], [1], [2], [2]
	BtsR-Phosphorylated	repressor	yobFp1	Sigma70	-207.0	-409.0	yobF, cspC	tttttttgctCATTCATCAATTtttcttattt nd	1907995	1908006	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	yobFp1	Sigma70	-174.0	-376.0	yobF, cspC	aaatttacaaTCCCTTTGGGGAttgacttctc nd	1907962	1907973	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]
	BtsR-Phosphorylated	repressor	yobFp1	Sigma70	-138.0	-340.0	yobF, cspC	gggtaattaaTAGCCGTTAACTgactgtttta nd	1907926	1907937	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-PATTERN-DISCOVERY], [EXP-GSELEX]	S	[1], [1]

Evolutionary conservation of regulatory elements

Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.

Promoter-target gene evolutionary conservation

Evidence
[EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis
[COMP-AINF-PATTERN-DISCOVERY] Automated inference based on sequence pattern discovery
[EXP-GSELEX] high throughput Genomic SELEX-chip evidence
[EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins
[EXP-IMP-SITE-MUTATION] Site mutation

Reference(s)
[1] Ogasawara H., Ishizuka T., Yamaji K., Kato Y., Shimada T., Ishihama A., 2019, Regulatory role of pyruvate-sensing BtsSR in biofilm formation by Escherichia coli K-12., FEMS Microbiol Lett 366(24)
[2] Kraxenberger T., Fried L., Behr S., Jung K., 2012, First insights into the unexplored two-component system YehU/YehT in Escherichia coli., J Bacteriol 194(16):4272-84
[3] Hirakawa H., Nishino K., Hirata T., Yamaguchi A., 2003, Comprehensive studies of drug resistance mediated by overexpression of response regulators of two-component signal transduction systems in Escherichia coli., J Bacteriol 185(6):1851-6
[4] Hirakawa H., Nishino K., Yamada J., Hirata T., Yamaguchi A., 2003, Beta-lactam resistance modulated by the overexpression of response regulators of two-component signal transduction systems in Escherichia coli., J Antimicrob Chemother 52(4):576-82

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