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HypT DNA-binding transcriptional dual regulator

Synonyms: HypT-Met-oxidized
Summary:
Based on microarray analysis it was shown that QseD, Quorum-sensing Escherichia coli regulator D, downregulates the flagellum regulon, decreasing motility [3]


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) References
HypT-Met-oxidized Functional   nd nd nd
Evolutionary Family: LysR
Connectivity class: Local Regulator
Gene name: hypT
  Genome position: 4557378-4558289
  Length: 912 bp / 303 aa
Operon name: hypT
TU(s) encoding the TF:
Transcription unit        Promoter
hypT
 


Regulon       
Regulated gene(s) cydA, cydB, cysH, fecA, fecB, fecC, fecD, fecE, metB, metI, metL, metN, metQ
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
membrane (5)
Fe aquisition (4)
ABC superfamily, membrane component (3)
electron acceptors (2)
ABC superfamily, periplasmic binding component (2)
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Regulated operon(s) cydAB, cysJIH, fecABCDE, metBL, metNIQ
First gene in the operon(s) cydA, cysH, fecA, metB, metN
Simple and complex regulons ArcA,FNR,H-NS,HypT
CRP,Fur,HypT,OxyR,PdhR
HypT
HypT,MetJ
HypT,MetJ,PhoP
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[HypT,-](1)
[HypT,+](4)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) References
  HypT-Met-oxidized activator cydAp1 Sigma70 nd nd cydA, cydB
nd
 nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] S [1], [1]
  HypT-Met-oxidized activator cysHp nd nd nd cysH
nd
 nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS] W [1], [2]
  HypT-Met-oxidized repressor fecAp nd nd nd fecA, fecB, fecC, fecD, fecE
nd
 nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS] W [1], [2]
  HypT-Met-oxidized activator metBp Sigma70 nd nd metB, metL
nd
 nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS] W [1], [2]
  HypT-Met-oxidized activator metNp Sigma70 nd nd metN, metI, metQ
nd
 nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] S [1], [1], [2], [2]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins


Reference(s)    

 [1] Gebendorfer KM., Drazic A., Le Y., Gundlach J., Bepperling A., Kastenmuller A., Ganzinger KA., Braun N., Franzmann TM., Winter J., 2012, Identification of a Hypochlorite-specific Transcription Factor from Escherichia coli., J Biol Chem 287(9):6892-903

 [2] Drazic A., Miura H., Peschek J., Le Y., Bach NC., Kriehuber T., Winter J., 2013, Methionine oxidation activates a transcription factor in response to oxidative stress., Proc Natl Acad Sci U S A 110(23):9493-8

 [3] Habdas BJ., Smart J., Kaper JB., Sperandio V., 2010, The LysR-type transcriptional regulator QseD alters type three secretion in enterohemorrhagic Escherichia coli and motility in K-12 Escherichia coli., J Bacteriol 192(14):3699-712


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